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Tiny paper cups filled with water and floating mosquito larvae occupied space in Aseel Rawashdeh's bedroom for months as she monitored the juvenile insects for changes in their behavior and structure. The 17-year-old was running an experiment: Would her inexpensive, eco-friendly concoction of baker's yeast and essential oils kill the wiggling larvae
In her research, Rawashdeh came across a 2014 study published in PLOS One about how entomologists at Louisiana State University created a bait using yeast. The bait would kill cellulose-digesting protozoa in the termite gut, leading to the termite's death. \"It was the first time the yeast capsule was used for insecticides, and I was pumped up, like what if I could apply this to something else What if I could apply it to mosquito larvae\" Rawashdeh gushes while explaining her research. Since some mosquitoes in the wild feed on yeast already, hiding a larvicide like essential oils within the yeast, she thought, could target specific species.
Rawashdeh knew from her readings that essential oils could act as a larvicide. Adding the yeast encapsulation made the oils more effective than if they were added to water with mosquito larvae on their own.
\"We know these essential oils will kill mosquitoes, but can we eliminate target impacts by just focusing on the mosquito and making them eat the essential oils The vast majority of mosquitoes, especially those that are a public health concern, feed on yeasts and algae matter in the water as juveniles,\" Schiller says.
Rawashdeh's experiment was novel in her method of encapsulating the essential oils into yeast, so that disease-spreading Aedes mosquitoes would ingest it while other organisms in the environment would not.
A paper published in Plant Metabolism and Chemodiversity in 2021 found that using essential oils derived from Piper species of plants as a larvicide was effective at targeting Aedes mosquitoes that had become resistant to pyrethroids. Pyrethroids are a type of pesticide used to control adult mosquitoes. Essential oils, however, encapsulate many compounds, and it is unknown which compounds in oils are causing mortality in larvae.
As soon as Rawashdeh confirmed that a yeast encapsulation could work with essential oils, she began to reach out to universities across the country to obtain various mosquito eggs for use in her experiments. In the summer of 2020, she received eggs from the Rockefeller strain of Aedes aegypti from Michael A. Riehle's entomology Lab at the University of Arizona. After countless emails sent across the country, Rawashdeh found Schiller, one of a few researchers in the United States who works closely with Toxorhynchites rutilus, or the mosquito assassin. These mosquitoes are dubbed \"mosquito assassins\" because they voraciously prey on other container-breeding mosquito species during their juvenile stage. When mosquito assassins grow into flying adults, they exclusively feed on flowers and do not require a blood meal. Schiller has worked to release mosquito assassins to help control other container-breeding mosquitoes that bite and take a blood meal from mammals in Harris County.
To create the homemade larvicide, Rawashdeh mixed essential oils with yeast and water and placed the medium into a shaking incubator, letting it soak for 10 hours at 45 degrees Celsius. \"I mainly tested the cheapest essential oils like peppermint, eucalyptus, orange and garlic. Those were just the most common ones and were reported to have some larvicidal activity in the literature,\" Rawashdeh says. While peppermint and eucalyptus were tested, Rawashdeh found in initial experiments that these oils did not work as well as the cinnamon, orange and garlic oils.
The results were promising. After the larvae fed on the bait, the essential oils inside caused neurotoxicity and mid-gut and tracheal damage, among other morphological changes not yet recorded in literature in larvae belonging to the Aedes mosquito species. Rawashdeh noted that some larvae died while others failed to grow into pupae.
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